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인플루엔자 바이러스 단일 클론 항 체를 중화의 탈출 변종의 생성
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
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인플루엔자 바이러스 단일 클론 항 체를 중화의 탈출 변종의 생성

11,172 Views

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07:55 min

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August 29, 2017

DOI:

10.3791/56067-v

DOI:
10.3791/56067-v

•

07:55 min
August 29, 2017

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, , , , , , ,
1Department of Microbiology,Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences,Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research,The University of Chicago

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Neutralizing AntibodiesMonoclonal AntibodiesEpitope MappingVirus Escape VariantsHemagglutination Inhibition (HI) AssayVirus NeutralizationEmbryonated Chicken EggsMDCK CellsVirus PassagingVirus-antibody Interactions

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Leon, P. E., Wohlbold, T. J., He, W., Bailey, M. J., Henry, C. J., Wilson, P. C., Krammer, F., Tan, G. S. Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies. J. Vis. Exp. (126), e56067, doi:10.3791/56067 (2017).

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