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הכנת הדוגמא לזיהוי מבוססי ספקטרומטר מסה של RNA-איגוד אזורים
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Bioquímica
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JoVE Journal Bioquímica
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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הכנת הדוגמא לזיהוי מבוססי ספקטרומטר מסה של RNA-איגוד אזורים

7,936 Views

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10:52 min

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September 28, 2017

DOI:

10.3791/56004-v

DOI:
10.3791/56004-v

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10:52 min
September 28, 2017

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1 Views
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1Epigenetics Institute,University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine

Tags

Sample PreparationMass SpectrometryRNA-binding ProteinsUV Cross-linking4sU LabelingCell FractionationImmunoprecipitationProtein-RNA InteractionsEpigeneticsCell CultureMouse Embryonic Stem Cells

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Warneford-Thomson, R., He, C., Sidoli, S., Garcia, B. A., Bonasio, R. Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions. J. Vis. Exp. (127), e56004, doi:10.3791/56004 (2017).

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