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Scansione retrovirale: Mappatura dei siti di integrazione MLV per definire regioni regolatori specifiche per le cellule

JoVE Journal
Genética

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JoVE Journal Genética

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

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Scansione retrovirale: Mappatura dei siti di integrazione MLV per definire regioni regolatori specifiche per le cellule

8,301 Views

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10:10 min

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May 28, 2017

DOI:

10.3791/55919-v

DOI:

10.3791/55919-v

•

10:10 min

May 28, 2017

•

2 Views

Oriana Romano², Ingrid Cifola, Valentina Poletti, Marco Severgnini, Clelia Peano, Gianluca De Bellis, Fulvio Mavilio⁴, Annarita Miccio^2,4,5

¹Center for Genome Research, Department of Life Sciences,University of Modena and Reggio Emilia, ²Laboratory of Chromatin and Gene Regulation During Development,Imagine Institute, ³Institute for Biomedical Technologies,CNR, ⁴Généthon, ⁵Sorbonne Paris Cité – Université Paris Descartes

Tags

Retroviral Scanning MLV Integration Sites Cell-specific Regulatory Regions Gene Therapy Gene Regulation MLV-derived Retroviral Vector EGFP Reporter Gene Flow Cytometric Analysis Genomic DNA Preparation Restriction Enzyme Digestion Linker Ligation First-round PCR Second-round PCR

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