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Isolamento dei mioblasti umani, valutazione della differenziazione myogenica e misurazione dell'entrata del calcio a livello di deposito
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
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Isolamento dei mioblasti umani, valutazione della differenziazione myogenica e misurazione dell'entrata del calcio a livello di deposito

9,813 Views

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10:45 min

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July 26, 2017

DOI:

10.3791/55918-v

DOI:
10.3791/55918-v

•

10:45 min
July 26, 2017

•

6 Views
, , ,
1Department of Orthopedic Surgery,Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences,Geneva Medical Center, 3Department of Cell Physiology and Metabolism,Geneva Medical Center

Tags

Human MyoblastsMyogenic DifferentiationStore-operated Calcium EntrySkeletal MuscleMuscle RegenerationIonic ChannelsTranscription FactorsMuscular DystrophiesTrypsinCell CultureCell IsolationCell Staining

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Laumonier, T., Koenig, S., Saüc, S., Frieden, M. Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement. J. Vis. Exp. (125), e55918, doi:10.3791/55918 (2017).

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