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Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
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Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
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07:51 min
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July 18, 2017
DOI:
1Department of Orthopedics, Beijing Chao-Yang Hospital,Capital Medical University, 2Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics,Chinese Academy of Sciences, 3Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology,Tongji University
Summary
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We present a protocol to generate a chondrogenic lineage from human peripheral blood (PB) via induced pluripotent stem cells (iPSCs) using an integration-free method, which includes embryoid body (EB) formation, fibroblastic cells expansion, and chondrogenic induction.
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Cite this Article
Li, Y., Hai, Y., Chen, J., Liu, T. Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells. J. Vis. Exp. (125), e55722, doi:10.3791/55722 (2017).
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