/
/
Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
8,944 Views
•
07:51 min
•
July 18, 2017
DOI:
1Department of Orthopedics, Beijing Chao-Yang Hospital,Capital Medical University, 2Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics,Chinese Academy of Sciences, 3Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology,Tongji University
Summary
Automatically generated
We present a protocol to generate a chondrogenic lineage from human peripheral blood (PB) via induced pluripotent stem cells (iPSCs) using an integration-free method, which includes embryoid body (EB) formation, fibroblastic cells expansion, and chondrogenic induction.
Read Article
Cite this Article
Li, Y., Hai, Y., Chen, J., Liu, T. Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells. J. Vis. Exp. (125), e55722, doi:10.3791/55722 (2017).
Copy