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发育生物学
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Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
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发育生物学
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JoVE 杂志 发育生物学
Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

DOI:
10.3791/55722-v

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07:51 min

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July 18, 2017

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, , ,
1Department of Orthopedics, Beijing Chao-Yang Hospital,Capital Medical University, 2Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics,Chinese Academy of Sciences, 3Clinical and Translational Research Center of Shanghai First Maternity & Infant Hospital, School of Life Sciences and Technology,Tongji University

Chapters

  • 00:05Title
  • 00:47Embryoid Body (EB) Formation
  • 03:37Cell Pellet Formation and Chondrocyte Differentiation
  • 05:42Results: Representative hiPSC-Chon Pellet Characterization
  • 06:54Conclusion

Summary

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We present a protocol to generate a chondrogenic lineage from human peripheral blood (PB) via induced pluripotent stem cells (iPSCs) using an integration-free method, which includes embryoid body (EB) formation, fibroblastic cells expansion, and chondrogenic induction.

Tags

ChondrocytesPeripheral BloodInduced Pluripotent Stem CellsIPS CellsCartilage RegenerationChondrogenic DifferentiationSerum-freeXeno-freeEmbryoid BodiesFibroblastic CellsGelatinBasal Culture Medium

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