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An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

DOI:
10.3791/55559-v

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12:17 min

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May 08, 2017

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, , , , ,
1Public Health Research Institute Centre, New Jersey Medical School,Rutgers, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, NIAID,NIH

Capítulos

  • 00:05Título
  • 01:02In Vitro Generation of Caseum Surrogate from THP-1 Cells
  • 05:23Rapid Equilibration Dialysis (RED) Assay
  • 08:02LC-MS Quantification and Data Analysis
  • 09:36Representative RED Assay Results
  • 11:27Conclusion

Summary

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Here we describe a rapid equilibrium dialysis (RED) method to measure drug binding to caseum from pulmonary tuberculosis lesions and cavities. The protocol is also used with a foamy macrophage-derived matrix that is an effective surrogate to caseum.

Tags

In Vitro Caseum Binding AssayDrug PenetrationTuberculosis LesionsAntimicrobial PenetrationTHP-1 MonocytesIliac AcidPMACell CultureRPMI Medium

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