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An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions
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JoVE Journal 免疫学と感染
An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

11,460 Views

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12:17 min

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May 08, 2017

DOI:

10.3791/55559-v

DOI:
10.3791/55559-v

•

12:17 min
May 08, 2017

•

5 Views
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1Public Health Research Institute Centre, New Jersey Medical School,Rutgers, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, NIAID,NIH

概要

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Here we describe a rapid equilibrium dialysis (RED) method to measure drug binding to caseum from pulmonary tuberculosis lesions and cavities. The protocol is also used with a foamy macrophage-derived matrix that is an effective surrogate to caseum.

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Cite this Article

Sarathy, J. P., Liang, H. H., Weiner, D., Gonzales, J., Via, L. E., Dartois, V. An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions. J. Vis. Exp. (123), e55559, doi:10.3791/55559 (2017).

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