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分离和新型表面标记组合来标识肝祖亚群富集
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
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分离和新型表面标记组合来标识肝祖亚群富集

DOI:
10.3791/55284-v

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08:52 min

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February 18, 2017

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, , , , ,
1Department of Medicine II,Saarland University Medical Center, 2Department of Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM),University of Saarland

Capítulos

  • 00:05Título
  • 00:58Liver Single Cell Suspension Preparation
  • 02:40Flow Cytometric Cell Count Determination and Cell Surface Staining
  • 04:30Magnetic Microbead-based Progenitor Cell Enrichment
  • 07:01Results: Representative Liver Progenitor Subset Analyses
  • 08:22Conclusion

Summary

Tadução automática

Tadução automática

肝损伤都伴随着祖细胞扩张表示异质细胞群。这种蜂窝室的新颖分级允许多个子集的区别。此处所描述的方法示出了流式细胞术分析，并且可用于进一步的测定各种子集的高纯度分离的流动。

Tags

Liver Progenitor CellsLiver Digestion ProtocolFlow CytometrySurface Marker CombinationCell IsolationCell BiologyHematopoietic CellsEndothelial CellsMouse LiverCell SuspensionCell CountingPropidium Iodide

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