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可诱导T7 RNA聚合酶介导的多基因表达系统，pMGX
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JoVE Journal Genética
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
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可诱导T7 RNA聚合酶介导的多基因表达系统，pMGX

DOI:
10.3791/55187-v

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10:09 min

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June 27, 2017

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, , ,
1Department of Chemistry and Biomolecular Sciences,University of Ottawa, 2School of Biosciences,The University of Nottingham Malaysia Campus

Capítulos

  • 00:05Título
  • 00:49Cloning Genes of Interest in the Multigene Expression System Vector, pMGX
  • 04:11Insert Gene 2 into the pMGX Vector Containing Gene 1, pMGX-yfg1
  • 07:08Results: Multigene Expression in pMGX
  • 09:10Conclusion

Summary

Tadução automática

Tadução automática

该研究描述了使用pMGX质粒系统在大肠杆菌中使用单个质粒的多个基因的T7介导的共表达的方法。

Tags

Inducible T7 RNA PolymeraseMultigene Expression SystemPMGXSynthetic BiologyBioquímicaProtein ComplexesCo-expressionEscherichia ColiGene CloningRestriction Enzyme DigestionGel ElectrophoresisGel ExtractionLigationBacterial TransformationAntibiotic Selection

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