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Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
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JoVE Journal Genetik
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

12,991 Views

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10:09 min

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June 27, 2017

DOI:

10.3791/55187-v

DOI:
10.3791/55187-v

•

10:09 min
June 27, 2017

•

18 Views
, , ,
1Department of Chemistry and Biomolecular Sciences,University of Ottawa, 2School of Biosciences,The University of Nottingham Malaysia Campus

Summary

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This study describes methods for the T7-mediated co-expression of multiple genes from a single plasmid in Escherichia coli using the pMGX plasmid system.

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Inducible T7 RNA PolymeraseMultigene Expression SystemPMGXSynthetic BiologyBiochemieProtein ComplexesCo-expressionEscherichia ColiGene CloningRestriction Enzyme DigestionGel ElectrophoresisGel ExtractionLigationBacterial TransformationAntibiotic Selection

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Cite this Article

Hassan, M. I., McSorley, F. R., Hotta, K., Boddy, C. N. Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX. J. Vis. Exp. (124), e55187, doi:10.3791/55187 (2017).

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