This study describes methods for the T7-mediated co-expression of multiple genes from a single plasmid in Escherichia coli using the pMGX plasmid system.
Hassan, M. I., McSorley, F. R., Hotta, K., Boddy, C. N. Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX. J. Vis. Exp. (124), e55187, doi:10.3791/55187 (2017).