É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
7,653 Views
•
10:40 min
•
December 28, 2016
DOI:
Summary
Automatically generated
Here, we present human cell culture protocols to analyze translation initiation factors that bind the 5' cap of mRNA during physiological oxygen conditions. This method utilizes an Agarose-linked m7GTP cap analog and is suitable to investigate cap-binding factors and their interacting partners.
Explore More Videos
Cap-binding ProteinsPhysiological Oxygen ConditionsAgarose Linked Seven Methyl-guanosine Cap AnalogCap-binding FactorsProtein SynthesisOxygen Regulated FactorsCap Dependent TranslationHypoxiaCancer TherapyTumor ProgressionNormaxiaCell CultureLysis BufferProtease Inhibitor Cocktail4-benzenesulfonyl Fluoride HydroclorideTrypsin EDTA
Read Article
Cite this Article
Timpano, S., Melanson, G., Evagelou, S. L., Guild, B. D., Specker, E. J., Uniacke, J. Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions. J. Vis. Exp. (118), e55112, doi:10.3791/55112 (2016).
Copy