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Journal / Genetics / Analysis of Cap-binding Proteins in Human Cells…
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Genetics

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JoVE Journal Genetics
Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
 

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Article DOI: 10.3791/55112-v • 10:40 min • December 28th, 2016
December 28th, 2016 •
*1, *1, 1, 1, 1, 1
1Department of Molecular and Cellular Biology, University of Guelph
* These authors contributed equally

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Summary
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Here, we present human cell culture protocols to analyze translation initiation factors that bind the 5' cap of mRNA during physiological oxygen conditions. This method utilizes an Agarose-linked m7GTP cap analog and is suitable to investigate cap-binding factors and their interacting partners.

Tags

Cap-binding Proteins Human Cells Physiological Oxygen Conditions Agarose Linked Seven Methyl-guanosine Cap Analog Cap-binding Factors Protein Synthesis Oxygen Regulated Factors Cap Dependent Translation Hypoxia Workstation Cancer Therapy Translational Control Tumor Progression Tissue Oxygenation Normaxia Cell Culture Complete Medium Cell Density
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