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Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

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10:40 min

December 28, 2016

DOI:

10:40 min
December 28, 2016

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Summary

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Here, we present human cell culture protocols to analyze translation initiation factors that bind the 5' cap of mRNA during physiological oxygen conditions. This method utilizes an Agarose-linked m7GTP cap analog and is suitable to investigate cap-binding factors and their interacting partners.

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