Journal
/
/
Analysis of Coronary Vessels in Cleared Embryonic Hearts
JoVE Journal
Biologia do Desenvolvimento
This content is Free Access.
JoVE Journal Biologia do Desenvolvimento
Analysis of Coronary Vessels in Cleared Embryonic Hearts

Analysis of Coronary Vessels in Cleared Embryonic Hearts

7,731 Views

08:25 min

December 07, 2016

DOI:

08:25 min
December 07, 2016

4 Views
, , ,

Summary

Automatically generated

We present a protocol for the analysis of coronary vessels in whole embryonic murine hearts up to E15.5, using standard immunological staining methods followed by optical clearance and confocal microscopy. This technique enables visualization of blood vessels throughout the entire heart without the need for time-consuming analysis of serial sections.

Read Article