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Analysis of Coronary Vessels in Cleared Embryonic Hearts
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December 07, 2016
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We present a protocol for the analysis of coronary vessels in whole embryonic murine hearts up to E15.5, using standard immunological staining methods followed by optical clearance and confocal microscopy. This technique enables visualization of blood vessels throughout the entire heart without the need for time-consuming analysis of serial sections.
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Ivins, S., Roberts, C., Vernay, B., Scambler, P. J. Analysis of Coronary Vessels in Cleared Embryonic Hearts. J. Vis. Exp. (118), e54800, doi:10.3791/54800 (2016).
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