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Analysis of Coronary Vessels in Cleared Embryonic Hearts
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Analysis of Coronary Vessels in Cleared Embryonic Hearts

Analysis of Coronary Vessels in Cleared Embryonic Hearts

DOI:
10.3791/54800-v

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08:25 min

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December 07, 2016

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, , ,
1Developmental Biology of Birth Defects,UCL Institute of Child Health, 2MRC Centre for Regenerative Medicine, SCRM Building,University of Edinburgh

章

  • 00:05標題
  • 00:51Embryonic Heart Dissection and Fixation
  • 02:07Embryonic Heart Whole Mount Immunostaining
  • 05:25Results: Representative Embryonic Heart Imaging
  • 07:11Conclusion

概要

自動翻訳

自動翻訳

We present a protocol for the analysis of coronary vessels in whole embryonic murine hearts up to E15.5, using standard immunological staining methods followed by optical clearance and confocal microscopy. This technique enables visualization of blood vessels throughout the entire heart without the need for time-consuming analysis of serial sections.

タグ

Coronary VesselsBABBCardiovascular DevelopmentCoronary Heart DiseaseEmbryonic HeartsImmunostainingWhole MountPrimary Antibody

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