Journal
/
Imunologia e Infecção
/
SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells

DOI:
10.3791/54799-v

•

10:24 min

•

March 03, 2017

•

, , , ,
1Brown University, 2COBRE Center for Cancer Research, Lifespan Laboratories,Rhode Island and Miriam Hospitals, 3Division of Infectious Diseases, Department of Medicine,Warren Alpert Medical School, Brown University

Capítulos

  • 00:05Título
  • 00:41Cell Culture and HIV-1 Infection
  • 02:11Exosome Isolation
  • 03:42Protein Extraction
  • 04:58Proteomic Data Analysis
  • 05:43Bioinformatics Verification and Characterization
  • 08:20Results: SILAC Is an Effective Method for Analyzing the Effects of HIV-1 Infection on Host Exosomal Proteomes
  • 09:08Conclusion

Summary

Tadução automática

Tadução automática

Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions.

Tags

Keywords: SILACProteomic CharacterizationExosomesHIV-1 InfectionCell CultureLabelingUltracentrifugationMass Spectrometry

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/54799?article_type=v

Specific Marking of HIV-1 Positive Cells using a Rev-dependent Lentiviral Vector Expressing the Green Fluorescent Protein
check_url/pt/54799?article_type=v

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
check_url/pt/54799?article_type=v

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
check_url/pt/54799?article_type=v

Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
check_url/pt/54799?article_type=v

In vitro Uncoating of HIV-1 Cores
check_url/pt/54799?article_type=v

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals
check_url/pt/54799?article_type=v

Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
check_url/pt/54799?article_type=v

Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
check_url/pt/54799?article_type=v

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals
check_url/pt/54799?article_type=v

Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1

Read Article