A protocol to screen for endocrine activity in organic extracts of water samples, including treated wastewater effluent and surface (receiving) water, was adapted using commercially available division-arrested ("freeze and thaw") in vitro transactivation bioassays.
Mehinto, A. C., Jayasinghe, B. S., Vandervort, D. R., Denslow, N. D., Maruya, K. A. Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays. J. Vis. Exp. (118), e54725, doi:10.3791/54725 (2016).