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Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays
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Journal JoVE Environnement
Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays

DOI:
10.3791/54725-v

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08:00 min

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December 04, 2016

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, , , ,
1Southern California Coastal Water Research Project Authority, 2Department of Physiological Sciences,University of Florida

Chapitres

  • 00:05Titre
  • 01:00Prepare Dilutions of the Assay-specific Reference Chemical and Water Extract
  • 02:42Prepare the Cell Suspension to Conduct the Fret Bioassay
  • 04:05Plate Cells in a 96-well Black Wall Clear-bottom Plate and Add Diluted Water Extract
  • 04:54Measure Cytotoxicity and Endocrine Activity Response
  • 05:51Résultats
  • 07:22Conclusion

Summary

Traduction automatique

Traduction automatique

A protocol to screen for endocrine activity in organic extracts of water samples, including treated wastewater effluent and surface (receiving) water, was adapted using commercially available division-arrested ("freeze and thaw") in vitro transactivation bioassays.

Tags

Keywords: Endocrine ActivityWater QualityIn Vitro Transactivation BioassaysReceptor-based Cell AssaysEndocrine Disrupting ChemicalsWater Sample PreparationDilution SeriesSolvent ControlCell ThawingCell Culture

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