Journal
/
Umwelt
/
Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays
JoVE Journal
Umwelt
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Umwelt
Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays

Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays

7,411 Views

•

08:00 min

•

December 04, 2016

DOI:

10.3791/54725-v

DOI:
10.3791/54725-v

•

08:00 min
December 04, 2016

•

2 Views
, , , ,
1Southern California Coastal Water Research Project Authority, 2Department of Physiological Sciences,University of Florida

Summary

Automatically generated

A protocol to screen for endocrine activity in organic extracts of water samples, including treated wastewater effluent and surface (receiving) water, was adapted using commercially available division-arrested ("freeze and thaw") in vitro transactivation bioassays.

Explore More Videos

Endocrine ActivityWater QualityIn Vitro Transactivation BioassaysReceptor-based Cell AssaysEndocrine Disrupting ChemicalsWater Sample PreparationDilution SeriesSolvent ControlCell ThawingCell Culture

Read Article

Cite this Article

Mehinto, A. C., Jayasinghe, B. S., Vandervort, D. R., Denslow, N. D., Maruya, K. A. Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays. J. Vis. Exp. (118), e54725, doi:10.3791/54725 (2016).

Download .ris file

Copy

Share Video

.

Copy