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A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
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Bioquímica
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JoVE Journal Bioquímica
A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

DOI:
10.3791/54722-v

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09:57 min

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December 23, 2016

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, ,
1Department of Biochemistry & Molecular Medicine,University of California, Davis, 2UC Davis Comprehensive Cancer Center,University of California, Davis, 3Department of Internal Medicine, Division of Hematology and Oncology,University of California, Davis

Capítulos

  • 00:05Título
  • 00:46Synthesis of TD PEG5K-Cys4-Ebes8-CA8
  • 03:57Preparation of PTX-loaded Micelles
  • 05:44Hemolysis Assay
  • 07:01Results: Characterizations of Reversible Disulfide Cross-linked Micelles
  • 08:51Conclusion

Summary

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Tadução automática

To deliver cancer drugs to tumor sites with high specificity and reduced side effects, new methods based on nanoparticles are required. Here, we describe disulfide cross-linked micelles that can be easily prepared by hydrogen peroxide-mediated oxidation and are able to dissociate efficiently under a reducing tumor environment to release payloads.

Tags

Disulfide Cross-linked MicellesDrug DeliveryNano-formulationsPolyethylene GlycolCholic AcidNN-DiisopropylethylamineDMFCentrifugationEther PrecipitationPolymer Synthesis

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