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A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
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Biochimie
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Journal JoVE Biochimie
A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

DOI:
10.3791/54722-v

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09:57 min

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December 23, 2016

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, ,
1Department of Biochemistry & Molecular Medicine,University of California, Davis, 2UC Davis Comprehensive Cancer Center,University of California, Davis, 3Department of Internal Medicine, Division of Hematology and Oncology,University of California, Davis

Chapitres

  • 00:05Titre
  • 00:46Synthesis of TD PEG5K-Cys4-Ebes8-CA8
  • 03:57Preparation of PTX-loaded Micelles
  • 05:44Hemolysis Assay
  • 07:01Results: Characterizations of Reversible Disulfide Cross-linked Micelles
  • 08:51Conclusion

Summary

Traduction automatique

Traduction automatique

To deliver cancer drugs to tumor sites with high specificity and reduced side effects, new methods based on nanoparticles are required. Here, we describe disulfide cross-linked micelles that can be easily prepared by hydrogen peroxide-mediated oxidation and are able to dissociate efficiently under a reducing tumor environment to release payloads.

Tags

Disulfide Cross-linked MicellesDrug DeliveryNano-formulationsPolyethylene GlycolCholic AcidNN-DiisopropylethylamineDMFCentrifugationEther PrecipitationPolymer Synthesis

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