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Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance

Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance

7,950 Views

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10:55 min

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October 16, 2018

DOI:

10.3791/54653-v

DOI:
10.3791/54653-v

•

10:55 min
October 16, 2018

•

1 Views
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1Clinical Microbiology, Department of Translational Medicine,Lund University, 2Department of Molecular Biology,Umea University

Summary

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This report describes protocols for characterizing interactions between bacterial outer membrane proteins and the human complement regulator vitronectin. The protocols can be used to study the binding reactions and biological function of vitronectin in any bacterial species.

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VitronectinBacterial AdhesionSerum ResistanceHost-pathogen InteractionComplement InhibitorFlow CytometryELISAAntihuman Vitronectin AntibodyFITC-conjugated AntibodyRecombinant PH Protein

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Singh, B., Mostajeran, M., Su, Y., Al-Jubair, T., Riesbeck, K. Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance. J. Vis. Exp. (140), e54653, doi:10.3791/54653 (2018).

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