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Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
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Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance
Chapitres
- 00:04Titre
- 00:49Detection of Vitronectin (Vn) Binding at the Bacterial Surface Using Flow Cytometry
- 02:18ELISA Analysis of the Interaction Between Recombinant Protein H (PH) and Vn
- 04:11Study of the Interaction Kinetics of Recombinant PH and Vn Using Bio-layer Interferometry
- 04:33Bacterial Adherence to the Vn-coated Glass Surfaces
- 05:32Vn-dependent Adherence of Bacteria to Epithelial Cells
- 07:29Analysis of Vn-dependent Resistance to the Bactericidal Activity of Human Serum
- 08:29Results: Interaction of Vitronectin and Bacteria
- 10:10Conclusion
This report describes protocols for characterizing interactions between bacterial outer membrane proteins and the human complement regulator vitronectin. The protocols can be used to study the binding reactions and biological function of vitronectin in any bacterial species.
