This report describes protocols for characterizing interactions between bacterial outer membrane proteins and the human complement regulator vitronectin. The protocols can be used to study the binding reactions and biological function of vitronectin in any bacterial species.
Singh, B., Mostajeran, M., Su, Y., Al-Jubair, T., Riesbeck, K. Assays for Studying the Role of Vitronectin in Bacterial Adhesion and Serum Resistance. J. Vis. Exp. (140), e54653, doi:10.3791/54653 (2018).