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荧光各向异性作为一种工具来研究蛋白质相互作用
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Bioquímica
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JoVE Journal Bioquímica
Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
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荧光各向异性作为一种工具来研究蛋白质相互作用

29,818 Views

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10:44 min

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October 21, 2016

DOI:

10.3791/54640-v

DOI:
10.3791/54640-v

•

10:44 min
October 21, 2016

•

14 Views
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1Departamento de Química de Biomacromoléculas, Instituto de Química,Universidad Nacional Autónoma de México, 2Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología,Universidad Nacional Autónoma de México

Summary

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蛋白质相互作用是在一个细胞的功能的心脏。量热和光谱技术通常用于表征它们。这里我们介绍荧光各向异性作为一种工具来研究在Shwachman-Diamond综合征（SBDS）突变蛋白质和延伸因子样1 GTP酶（EFL1）之间的相互作用。

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Gijsbers, A., Nishigaki, T., Sánchez-Puig, N. Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions. J. Vis. Exp. (116), e54640, doi:10.3791/54640 (2016).

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