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抗体标记用荧光染料使用磁蛋白A和G蛋白珠
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
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抗体标记用荧光染料使用磁蛋白A和G蛋白珠

DOI:
10.3791/54545-v

•

06:48 min

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September 15, 2016

•

, ,
1Promega Corporation

Capítulos

  • 00:05Título
  • 00:41High Capacity Magnetic Protein A or G Bead Antibody Capture
  • 01:43Antibody Labeling Using Amine Chemistry
  • 02:56Antibody Labeling Using Thiol Chemistry
  • 04:46Results: Magnetic BEad Fluorescent Dye Labeling Results in a Highly Efficient Purified Antibody Recovery
  • 05:39Conclusion

Summary

Tadução automática

Tadução automática

上的胎圈的方法用于标记抗体与小分子使抗体的少量的标记直接从细胞培养基。这种方法是与胺和硫醇化学相容，并且可以并行处理多个样品，手工或使用自动平台。

Tags

Antibody LabelingFluorescent DyesMagnetic Protein A BeadsProtein G BeadsAmine ChemistryThiol ChemistryAntibody PurificationCell MediumAutomated LabelingManual LabelingAbsorbance MeasurementConjugation BufferElution BufferNeutralization Buffer

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