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抗体标记用荧光染料使用磁蛋白A和G蛋白珠
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
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抗体标记用荧光染料使用磁蛋白A和G蛋白珠

11,556 Views

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06:48 min

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September 15, 2016

DOI:

10.3791/54545-v

DOI:
10.3791/54545-v

•

06:48 min
September 15, 2016

•

19 Views
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Summary

Automatically generated

上的胎圈的方法用于标记抗体与小分子使抗体的少量的标记直接从细胞培养基。这种方法是与胺和硫醇化学相容，并且可以并行处理多个样品，手工或使用自动平台。

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Cite this Article

Nath, N., Godat, B., Urh, M. Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads. J. Vis. Exp. (115), e54545, doi:10.3791/54545 (2016).

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