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抑制接枝反应性免疫的调节性巨噬细胞的功能表征
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity
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抑制接枝反应性免疫的调节性巨噬细胞的功能表征

DOI:
10.3791/54242-v

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08:41 min

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June 07, 2017

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,
1Department of Medicine,Icahn School of Medicine at Mount Sinai, 2Immunología de Trasplantes, Centro Nacional de Microbiología,Instituto de Salud Carlos III

Capítulos

  • 00:05Título
  • 00:45Graft-infiltrating Macrofage Isolation by Fluorescence-activated Cell Sorting (FACS)
  • 03:23T Cell Isolation by FACS
  • 06:39Results: Representative In Vitro Macrophage Subset Suppressive Capacity Analyses
  • 07:36Conclusion

Summary

Tadução automática

Tadução automática

巨噬细胞是造血系统的塑料细胞，在保护性免疫和体内平衡方面起关键作用。在本报告中，我们描述了优化的体外技术，以表型和功能表征在耐受性条件下积累在移植器官中的移植物浸润调节性巨噬细胞。

Tags

MacrophagesRegulatory MacrophagesGraft-reactive ImmunityAllograftSingle-cell SuspensionFlow CytometryCell SortingCD11bCD45Ly6CLy6GDAPIViabilityCell Culture

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