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JoVE Journal
Immunology and Infection

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Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity
 

Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity

Article DOI: 10.3791/54242-v 08:41 min June 7th, 2017
June 7th, 2017

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résumé

Macrophages are plastic cells of the hematopoietic system that have a crucial role in protective immunity and homeostasis. In this report, we describe optimized in vitro techniques to phenotypically and functionally characterize graft-infiltrating regulatory macrophages that accumulate in the transplanted organ under tolerogenic conditions.

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Keywords: Macrophages Regulatory Macrophages Graft-reactive Immunity Allograft Single-cell Suspension Flow Cytometry Cell Sorting CD11b CD45 Ly6C Ly6G DAPI Viability Cell Culture
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