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Immunology and Infection

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JoVE Journal Immunology and Infection
Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity
 

Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity

Article DOI: 10.3791/54242-v • 08:41 min • June 7th, 2017
June 7th, 2017 •
1,2, 1,2
1Department of Medicine, Icahn School of Medicine at Mount Sinai, 2Immunología de Trasplantes, Centro Nacional de Microbiología, Instituto de Salud Carlos III

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Resumen
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Macrophages are plastic cells of the hematopoietic system that have a crucial role in protective immunity and homeostasis. In this report, we describe optimized in vitro techniques to phenotypically and functionally characterize graft-infiltrating regulatory macrophages that accumulate in the transplanted organ under tolerogenic conditions.

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Functional Characterization Regulatory Macrophages Graft-reactive Immunity In Vitro Procedure Macrophage Populations Allografted Hearts Mass Transplantation Model Allograph Survival Tolerance Specific Tissue Infiltrated Cardiac Allograph Isolated Microphages Subset Host Tissue Response Single-cell Suspension FC Receptor Blocking Monoclonal Antibody Myeloid Cell Surface Antibodies RPMI Medium Trypan Blue 10 To The Sixth Cells Per Milliliter Concentration 70 Micron Strainer DAPI Viability Marker Flow Cytometer Macrophage Sorting Parameters
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