Macrophages are plastic cells of the hematopoietic system that have a crucial role in protective immunity and homeostasis. In this report, we describe optimized in vitro techniques to phenotypically and functionally characterize graft-infiltrating regulatory macrophages that accumulate in the transplanted organ under tolerogenic conditions.
Ochando, J., Conde, P. Functional Characterization of Regulatory Macrophages That Inhibit Graft-reactive Immunity. J. Vis. Exp. (124), e54242, doi:10.3791/54242 (2017).