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Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy
 

Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy

Article DOI: 10.3791/54214-v • 07:47 min • July 9th, 2016
July 9th, 2016 •
1, 1, 1, 2, 2, 1
1Virginia Tech Carilion Research Institute, 2Renovo Neural Incorporated

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Mitochondrial visualization and analysis from mammalian brain tissue is a challenging task. Here, we describe how three dimensional (3D) reconstruction analysis from the serial block-face scanning electron microscopy (SBFSEM) can be used to gain insights on the morphological and volumetric analysis of this critical energy generating organelle.

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Serial Block-face Scanning Electron Microscopy Brain Mitochondria Morphology Structure Distribution Volume Number Neurobiology Neurodegenerative Disorders Neurodevelopmental Disorders Custom Ultramicrotome Glutaraldehyde-fixed Tissues Cacodylate Buffer Tannic Acid Potassium Ferrocyanide Sodium Cacodylate Osmium Tetroxide Osmium Ferrocyanide Solution Thiocarbohydrazide TCH Solution
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