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初级量子神经元轴突运输的实时成像点标记的BDNF

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

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初级量子神经元轴突运输的实时成像点标记的BDNF

DOI:

10.3791/51899-v

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10:53 min

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September 15, 2014

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Xiaobei Zhao, Yue Zhou, April M. Weissmiller, Matthew L. Pearn⁴, William C. Mobley, Chengbiao Wu

¹Department of Neurosciences,University of California, San Diego, ²School of Biomedical Engineering and Med-X Research Institute,Shanghai Jiao Tong University, ³Department of Anesthesiology,University of California, San Diego, ⁴VA San Diego Healthcare System

Capítulos

00:05Título
01:32Preparation of Microfluidic Chambers
03:26Dissection of Neuronal Culture and Plating on Chambers
05:41Axonal Transport of Quantum Dot-labeled Brain-derived Neurotrophic Factor (QD-BDNF)
07:43Results: Live Imaging of Axonal Transport of QD-BDNF in Hippocampal Neurons
10:27Conclusion

Summary

Tadução automática

BDNF，神经营养因子，对轴突运输是几个神经元群体的生存和功能是至关重要的。一些退行性疾病的标志是轴索结构和功能的破坏。我们展示了用于检查QD-BDNF在使用初级神经元微流控室现场贩卖的技术。

Tags

Axonal Transport BDNF Quantum Dot Neurodegenerative Disease Microfluidic Device Retrograde Transport Axonal Function

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