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Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals
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Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals

Multimodal Optical Microscopy Methods Reveal Polyp Tissue Morphology and Structure in Caribbean Reef Building Corals

DOI:
10.3791/51824-v

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10:39 min

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September 05, 2014

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, , ,
1Institute for Genomic Biology,University of Illinois at Urbana-Champaign, 2Department of Geology,University of Illinois at Urbana-Champaign, 3Department of Microbiology,University of Illinois at Urbana-Champaign

Capítulos

  • 00:05Título
  • 01:04Wax Preinfiltration and Embedding
  • 06:18Imaging Corals Under Two-photon Fluorescence Microscopy
  • 07:293D Volume Rendering and Visualization of SBFI and Two-photon Spectral Fluorescence Data
  • 08:55Results: Two-photon Fluorescence 3D Images of Coral Polyps
  • 09:58Conclusion

Summary

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An integrated suite of imaging techniques has been applied to determine polyp morphology and tissue structure in the Caribbean corals Montastraeaannularis and M. faveolata. Fluorescence, serial block face, and two-photon confocal laser scanning microscopy have identified lobate structure, polyp walls, and estimated chromatophore and zooxanthellae densities and distributions.

Tags

Keywords: Multimodal Optical MicroscopyReef Building CoralsMontastraea AnnularisMontastraea FaveolataFluorescence MicroscopySerial Block Face ImagingTwo-photon Confocal Laser Scanning MicroscopyChromatophoresZooxanthellaeChlorophyll

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