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Immunology and Infection

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JoVE Journal Immunology and Infection
Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
 

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Article DOI: 10.3791/51646-v • 09:14 min • June 13th, 2014
June 13th, 2014 •
1, 1, 1, 1,2
1Department of Medicine, New York University School of Medicine, 2Departments of Pathology, New York University School of Medicine

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Static adhesion assay is a powerful tool that can be used to model the interactions between T lymphocytes and other cell types. Interactions are generated by injecting labeled T cells into wells coated with adhesion molecules, while a plate reader is used to quantify the number of adherent cells following serial washes.

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Static Adhesion Assay Integrin Activation T Lymphocytes T Cell Functions Migration Immunological Synapses Cell Adhesion Molecules Integrins Lymphocyte Function-associated Antigen (LFA)-1 Intracellular Adhesion Molecule (ICAM)-1 Affinity States Inside-out Signaling Mechanistic Studies In Vitro Model Systems Fluorescent Plate Reader Adhesiveness Adhesion-stimulatory Substances Inhibitory Substances Signaling Events
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