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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes
 

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Article DOI: 10.3791/50765-v • 12:59 min • September 26th, 2013
September 26th, 2013 •
*1, *1, 1, 1, 1
1Department of Microbiology and Cell Science, The University of Florida
* These authors contributed equally

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With effector functions distinct from other T cell subsets, Th17 cells have been centrally implicated in inflammatory autoimmunity. This in vitro Th17 differentiation protocol provides a means to determine whether naïve CD4+ T lymphocytes can differentiate into Th17 cells, and to further examine their role in autoimmunity and host response.

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Isolation Th17 Differentiation Naïve CD4 T Lymphocytes IL-17 T Cell Subsets Autoimmune Disorders Purification Spleen Lymph Nodes CD4+ T Cells Th17-inducing Environment Anti-CD3 Anti-CD28 Antibodies IL-6 TGF-β Incubation Flow Cytometry QPCR ELISAs CD4+CD25- T Cells Autoimmunity Host Defense Murine Knockout/disease Models
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