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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation and Activation of Murine Lymphocytes
 

Isolation and Activation of Murine Lymphocytes

Article DOI: 10.3791/54596-v • 08:08 min • October 30th, 2016
October 30th, 2016 •
1, 1, 1
1School of Biological Sciences, College of Science, Nanyang Technological University

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Resumo
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Lymphocytes are the major players in adaptive immune responses. Here, we present a lymphocyte purification protocol to determine the physiological functions of the desired molecules in lymphocyte activation in vitro and in vivo. The described experimental procedures are suitable for comparing functional capacities between control and genetically modified lymphocytes.

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Isolation Activation Murine Lymphocytes Functional Assays Immunology Molecular Biology Protein Of Interest Gene Deficient Lymphocytes Purification B Cells Red Blood Cell Lysis Balanced Salt Solution FBS Anti-CD43 Magnetic Micro Beads Annexin Five Magnetic Beads Centrifugation Separation Column Magnetic Rack
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