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An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila
JoVE Journal
Neurociência
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JoVE Journal Neurociência
An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila

13,099 Views

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10:49 min

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March 28, 2013

DOI:

10.3791/50306-v

DOI:
10.3791/50306-v

•

10:49 min
March 28, 2013

•

7 Views
,
1Neurodevelopment Group, School of Biosciences,University of Birmingham

Summary

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An injury paradigm using the Drosophila larval ventral nerve cord to investigate central nervous system regeneration and repair is described. Stabbing followed by laser scanning confocal microscopy in time-lapse and fixed specimens, combined with quantitative analysis with purposefully developed software and genetics, are used to investigate the molecular mechanisms of CNS regeneration and repair.

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Central Nervous SystemCNSInjuryRepairRegenerationDrosophilaVentral Nerve CordVNCSpinal CordLaser Scanning Confocal MicroscopyImmunofluorescenceImageJCell ProliferationCell DeathGenéticaMolecular Mechanisms

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Cite this Article

Kato, K., Hidalgo, A. An Injury Paradigm to Investigate Central Nervous System Repair in Drosophila. J. Vis. Exp. (73), e50306, doi:10.3791/50306 (2013).

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