/
/
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
16,772 Views
•
08:17 min
•
August 23, 2013
DOI:
Summary
Automatically generated
Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.
Read Article
Cite this Article
Senthil-Kumar, M., Lee, H., Mysore, K. S. VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance. J. Vis. Exp. (78), e51033, doi:10.3791/51033 (2013).
Copy