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VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

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08:17 min

August 23, 2013

DOI:

08:17 min
August 23, 2013

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概要

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Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.

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