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VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
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VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
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August 23, 2013
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Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.
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Senthil-Kumar, M., Lee, H., Mysore, K. S. VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance. J. Vis. Exp. (78), e51033, doi:10.3791/51033 (2013).
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