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体外共生分析评估病原体诱导中性粒细胞跨上皮迁移

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JoVE Journal Imunologia e Infecção

In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration

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体外共生分析评估病原体诱导中性粒细胞跨上皮迁移

DOI:

10.3791/50823-v

•

14:14 min

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January 06, 2014

•

Mark E. Kusek^2,3, Michael A. Pazos³, Waheed Pirzai, Bryan P. Hurley³

¹Department of Pediatrics,Harvard Medical School, ²Department of Pediatric Gastroenterology,MGH for Children, ³Mucosal Immunology and Biology Research Center,Massachusetts General Hospital

Capítulos

00:05Título
02:01Collagen Coating Transwells
03:25Seeding Collagen Coated Transwells with Epithelial Cells
04:30Preparation of Epithelial Cell Layers for Migration Assay
06:01Neutrophil Trans-epithelial Migration Assay
11:27Results: Assessing Neutrophil Trans-epithelial Migration
13:23Conclusion

Summary

Tadução automática

中性粒细胞跨上皮迁移，以应对粘膜细菌感染，有助于上皮损伤和临床疾病。已经开发出一种体外模型，结合了病原体、人类嗜中性粒细胞和在转井过滤器上生长的极化人类上皮细胞层，以促进研究，以解开协调这一现象的分子机制。

Tags

In Vitro Coculture Neutrophil Trans-epithelial Migration Mucosal Immunity Pathogenic Infection Polarized Epithelial Monolayer Neutrophil Infiltration Inflammatory Response Therapeutic Targets

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