/
/
Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat
2,340 Views
•
04:30 min
•
February 04, 2021
DOI:
1Department of Cardiology, Campus Benjamin Franklin,Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), 2Department of Experimental and Clinical Medicine,University of Florence, 3Berlin Institute of Health, 4Department of Cardiology,German Heart Centre Berlin (DHZB), 5Sod of Interdisciplinary Internal Medicine,Azienda Ospedaliera Universitaria Careggi (AOUC), 6Department of General and Interventional Cardiology,Helios Klinikum Erfurt
Summary
Automatically generated
In this study, we describe the process of T lymphocyte isolation from fresh samples of calcified aortic valves and the analytical steps of T cell-cloning for the characterization of the adaptive leukocyte subsets by using flow cytometry analysis.
Read Article
Cite this Article
Curini, L., Christopher, M. R., Grubitzsch, H., Landmesser, U., Amedei, A., Lauten, A., Alushi, B. Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat. J. Vis. Exp. (168), e62059, doi:10.3791/62059 (2021).
Copy