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Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
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Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
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June 25, 2015
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The method described here is used to induce the apoptotic signaling cascade at defined steps in order to dissect the activity of an anti-apoptotic bacterial effector protein. This method can also be used for inducible expression of pro-apoptotic or toxic proteins, or for dissecting interference with other signaling pathways.
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Berens, C., Bisle, S., Klingenbeck, L., Lührmann, A. Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling. J. Vis. Exp. (100), e52903, doi:10.3791/52903 (2015).
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