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Imunologia e Infecção
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Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

DOI:
10.3791/57287-v

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09:09 min

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April 20, 2018

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, , ,
1Norton Thoracic Institute,St. Joseph’s Hospital and Medical Center, 2Flow Cytometry Core,St. Joseph’s Hospital and Medical Center

Capítulos

  • 00:04Título
  • 00:50Isolation of Alveolar Macrophages (AMs) from Murine Bronchoalveolar Lavage (BAL) Fluid
  • 03:58Isolation of AMs from Mouse Lung: Single-cell Suspension
  • 06:04In Vitro Culture of AMs
  • 07:06Results: Characteristics of Mouse Alveolar Macrophages
  • 07:42Conclusion

Summary

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Tadução automática

This communication describes methodologies for isolation and culture of alveolar macrophages from humans and murine models for experimental purposes.

Tags

Alveolar MacrophagesIn Vitro CultureMurineHumanLungBronchoalveolar LavagePulmonary ImmunologyRespiratory InfectionInflammationAutoimmunityCell IsolationTracheal CatheterizationPerfusion

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