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隔离和脂质A的化学表征革兰氏阴性菌

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Química

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JoVE Journal Química

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria

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隔离和脂质A的化学表征革兰氏阴性菌

DOI:

10.3791/50623-v

•

12:57 min

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September 16, 2013

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Jeremy C. Henderson, John P. O’Brien, Jennifer S. Brodbelt, M. Stephen Trent³

¹Section of Molecular Genetics and Microbiology,The University of Texas at Austin, ²Department of Chemistry and Biochemistry,The University of Texas at Austin, ³The Institute of Cellular and Molecular Biology,The University of Texas at Austin

Capítulos

00:05Título
02:00Large Scale Lipid A Extraction (50 ml to 1.5 L)
06:22Electrospray Ionization Mass Spectrometry of Lipid A and Collision Induced Dissociation
07:56MS/MS on Lipid A by Ultraviolet Photodissociation (UVPD)
09:04Results: Chemical Characterization of Lipid A by Mass Spectrometry and Thin Layer Chromatography
12:00Conclusion

Summary

Tadução automática

隔离脂多糖（LPS）由革兰氏阴性菌的类脂A结构域和表征提供了洞察抗生素耐药性的细胞表面为基础的机制，细菌的生存和健康，以及如何化学性质不同的脂质分子种类差异调节宿主先天免疫反应。

Tags

Keywords: Lipopolysaccharide Lipid A Gram-negative Bacteria Cell Surface Molecule Chemical Characterization Mass Spectrometry Thin Layer Chromatography Innate Immune Response Antibiotic Resistance

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