/
/
Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
12,005 Views
•
12:38 min
•
September 16, 2013
DOI:
Summary
Automatically generated
Human neuroprogenitor cells (NPCs) were expanded under proliferating conditions. NPCs were differentiated into neuron-rich cultures in the presence of a combination of neurotrophins. Neuronal markers were detected by immunofluorescence staining. To isolate a pure population of neurons, NPCs were differentiated on PEN membrane slides and laser capture microdissection was performed.
Read Article
Cite this Article
Bouchard, R., Chong, T., Pugazhenthi, S. Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture. J. Vis. Exp. (79), e50487, doi:10.3791/50487 (2013).
Copy